Nuclear localization of the testis determining gene product SRY.
نویسندگان
چکیده
منابع مشابه
Nuclear localization of the testis determining gene product SRY
We have studied the expression of the human SRY protein (termed p27SRY) in two different cell lines by using specific antibodies. Confocal microscopy enabled us to localize p27SRY precisely in the nucleus in a discrete punctuate pattern. Furthermore, through microinjection experiments, we have demonstrated that the localization of the p27SRY protein into the nucleus was an event involving the N...
متن کاملDistribution of the molossinus allele of Sry, the testis-determining gene, in wild mice.
When the Y chromosome of the laboratory inbred mouse strain C57BL/6 (B6) is replaced by the Y of certain strains of Mus musculus domesticus, testis determination fails and all XY fetuses develop either as hermaphrodites or XY females (XY sex reversal). This suggests the presence of at least two alleles of Sry, the male-determining gene on the Y:M. m. domesticus and B6. The B6 Y chromosome is de...
متن کاملExpression of Sry, the mouse sex determining gene.
In the mouse, Sry is expressed by germ cells in the adult testis and by somatic cells in the genital ridge. Transcripts in the former exist as circular RNA molecules of 1.23 kb, which are unlikely to be efficiently translated. We have used RNase protection to map the extent of the less abundant Sry transcript in the developing gonad. We demonstrate that it is a linear mRNA derived from a single...
متن کاملGlobal Genome Analysis of the Downstream Binding Targets of Testis Determining Factor SRY and SOX9
A major event in mammalian male sex determination is the induction of the testis determining factor Sry and its downstream gene Sox9. The current study provides one of the first genome wide analyses of the downstream gene binding targets for SRY and SOX9 to help elucidate the molecular control of Sertoli cell differentiation and testis development. A modified ChIP-Chip analysis using a comparat...
متن کاملNMR spectroscopic analysis of the DNA conformation induced by the human testis determining factor SRY.
The conformation of an eight base pair DNA oligonucleotide duplex bound to the human testis determining factor SRY and the orientation of the protein domain within the complex have been analyzed by a variety of NMR methods which permit the selective observation of protons attached to 12C nuclei in the presence of uniformly enriched 13C/15N protein. Qualitative analysis of nuclear and rotating f...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1995
ISSN: 0021-9525,1540-8140
DOI: 10.1083/jcb.128.5.737